DNA Typing Compatibility with a One Step Saliva Screening Test

A novel one-step Helicobacter pylori saliva antigen test.

BACKGROUND A rapid, reliable, and sufficiently accurate test for diagnosing Helicobacter pylori infection is required for screening dyspeptic patients before a referral for endoscopy. The purpose of this article is two-fold: first, to evaluate the accuracy of a one-step H. pylori saliva antigen (HPS) test; and second, to compare noninvasive and invasive H. pylori tests in Taiwanese population. ...

One step forward

Viral DNA Packaging: One Step at a Time

During its life-cycle the bacteriophage ϕ29 actively packages its dsDNA genome into a proteinacious capsid, compressing its genome to near crystalline densities against large electrostatic, elastic, and entropic forces. This remarkable process is accomplished by a nano-scale, molecular DNA pump – a complex assembly of three protein and nucleic acid rings which utilizes the free energy released ...

Unusual Case Leads to DNA Screening Test

One-step RT-PCR for screening microdissected tissue samples.

The technology of laser-capture microdissection (LCM) enables the easy extraction of single cells or defined groups of cells from a tissue section (1,2). This technique uses a laser pulse to activate a plastic polymer, which binds to and captures cells from a frozen or paraffin-embedded tissue section. Captured cells present on this solid support can then be used for nucleic acid and/or protein...